Thiosemicarbazones, thiosemicarbazides, thioureas and also isothiocyanates are made to react with 2,3-dichloro-1,4-naphthoquinone in an ethanolic medium and on rendering the mixtures alkaline with ethanolic ammonia solution, a red to violet colour is developed with absorption maxima between 500 and 570 nm. The reaction forms the basis of a spot test for these compounds.
M. B. Devani, C. J. Shishoo and M. G. Shah, Analyst, 1973, 98, 759 DOI: 10.1039/AN9739800759
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