In the 13C spectrum of nicotinamide adenine dinucleotide (NAD+) the adenine and nicotinamide ring carbons are assigned by a graphical method of presenting the proton off-resonance decoupled 13C spectra as a function of proton irradiation frequency; this method is particularly applicable to closely spaced 13C signals.
B. Birdsall, N. J. M. Birdsall and J. Feeney, J. Chem. Soc., Chem. Commun., 1972, 316 DOI: 10.1039/C39720000316
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