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Enhanced photostability of cyanine fluorophores across the visible spectrum

Nature Methods volume 9, pages 428–429 (2012)Cite this article

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A Corrigendum to this article was published on 30 May 2012

This article has been updated

To the Editor:

Our recent demonstration1 that proximally linked 'protective' agents can yield substantial improvements in the performance of the cyanine fluorophore Cy5 motivated us to develop more photostable fluorophores spanning the visible spectrum. Such fluorophores would enable a broad range of applications and experimental demands. Here, using strategies identical to those previously described1, we show that marked enhancements in performance can also be achieved for other widely used members of the cyanine class, Cy2, Cy3, Cy3.5, Cy5.5 and Cy7 (Fig. 1a) when coupled to individual cyclooctatetraene (COT), nitrobenzylalcohol (NBA) and Trolox molecules (Supplementary Methods).

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Figure 1: Enhanced performance of distinct cyanine-class fluorophores through the direct conjugation to protective agents.

Change history

  • 27 April 2012

    In the version of this article initially published, the y-axis label on a graph in Figure 1b was incorrect. The error has been corrected in the HTML and PDF versions of the article.

References

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Acknowledgements

We thank the members of the Blanchard laboratory for their critical comments on the manuscript. This work was supported by US National Institutes of Health (3R01GM079238-06S1 to S.C.B.), the Tri-Institutional Training Program in Chemical Biology and the Tri-Institutional Training Program in Computational Biology and Medicine.

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Author notes

  1. Roger B Altman and Qinsi Zheng: These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Physiology and Biophysics, Weill Medical College of Cornell University, New York, New York, USA

    Roger B Altman, Qinsi Zheng, Zhou Zhou, Daniel S Terry & Scott C Blanchard

  2. Tri-Institutional Training Program in Chemical Biology, Weill Medical College of Cornell University, New York, New York, USA

    Qinsi Zheng & Scott C Blanchard

  3. Tri-Institutional Training Program in Computational Biology and Medicine, Weill Medical College of Cornell University, New York, New York, USA

    Daniel S Terry & Scott C Blanchard

  4. Department of Biochemistry, Weill Medical College of Cornell University, New York, New York, USA

    J David Warren & Scott C Blanchard

Authors
  1. Roger B Altman
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  2. Qinsi Zheng
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  3. Zhou Zhou
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  4. Daniel S Terry
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  5. J David Warren
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  6. Scott C Blanchard
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Corresponding author

Correspondence to Scott C Blanchard.

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The authors declare no competing financial interests.

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Altman, R., Zheng, Q., Zhou, Z. et al. Enhanced photostability of cyanine fluorophores across the visible spectrum. Nat Methods 9, 428–429 (2012). https://doi.org/10.1038/nmeth.1988

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