Thiophilic gels: an alternative to protein A and G for use in flow injection immunoassays

Abstract

Simple fluorescence flow injection immunoassays have been developed for theophylline using a thiophilic affinity matrix (T-gel). This gel is far superior to the previously used bacterial immunoglobulin binding proteins (e.g., proteins A and G) in terms of cost, elution conditions, non-specific binding and specificity for immunoglobulins regardless of type or subclass.