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Issue 2, 2015
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A modified weight-of-evidence approach to evaluate the allergenic potential of food proteins

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Abstract

Assessment of the allergenic potential of food proteins, including novel proteins, is an important issue. However, the current weight-of-evidence approach cannot provide a direct evaluation of the inherent allergenic potential of food proteins. In order to make up for this deficiency, we made some supplements or modifications to develop a more comprehensive strategy, involving consideration of the epitopes of allergens, biochemical characterization (i.e., resistance to digestion in simulated gastric fluid, heat stability), human serum analysis, and appropriate cell models and animal models to evaluate the allergenic potential of food proteins. Results indicated that the bioinformatics used can directly predict the linear epitopes of food allergens in addition to the sequence homology comparison. Human serum studies may assess the clinical reactivity of food allergens based on the combination of the specific IgE/IgG4 ratio and the specific IgE levels. Further, an RBL cell-based immunoassay is applied to explore functional IgE–allergen interactions. The final step consists of dissecting the mechanism behind the allergenicity of food proteins by animal studies. In conclusion, we reported a modified weight-of-evidence approach to evaluate the allergenic potential of food proteins but not novel proteins. This modified approach provides an integrated, stepwise and direct evaluation of the allergenic potential of a wider range of food proteins.

Graphical abstract: A modified weight-of-evidence approach to evaluate the allergenic potential of food proteins

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Publication details

The article was received on 19 Nov 2014, accepted on 06 Jan 2015 and first published on 07 Jan 2015


Article type: Paper
DOI: 10.1039/C4TX00211C
Toxicol. Res., 2015,4, 476-485

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    A modified weight-of-evidence approach to evaluate the allergenic potential of food proteins

    N. Sun, L. Tekutyeva, S. Wang, Q. Pu, C. Zhou, J. Wang and H. Che, Toxicol. Res., 2015, 4, 476
    DOI: 10.1039/C4TX00211C

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