Jump to main content
Jump to site search

Issue 41, 2020
Previous Article Next Article

Rapid and cytocompatible cell-laden silk hydrogel formation via riboflavin-mediated crosslinking

Author affiliations

Abstract

Bioactive hydrogels based on naturally-derived polymers are of great interest for regenerative medicine applications. Among naturally-derived polymers, silk fibroin has been extensively explored as a biomaterial for tissue engineering due to its unique mechanical properties. Here, we demonstrate the rapid gelation of cell-laden silk fibroin hydrogels by visible light-induced crosslinking using riboflavin as a photo-initiator, in presence of an electron acceptor. The gelation kinetics were monitored by in situ photo-rheometry. Gelation was achieved in minutes and could be tuned owing to its direct proportionality to the electron acceptor concentration. The concentration of the electron acceptor did not affect the elastic modulus of the hydrogels, which could be altered by varying the polymer content. Further, the biocompatible riboflavin photo-initiator combined with sodium persulfate allowed for the encapsulation of cells within silk fibroin hydrogels. To confirm the cytocompatibility of the silk fibroin formulations, three cell types (articular cartilage-derived progenitor cells, mesenchymal stem cells and dental-pulp-derived stem cells) were encapsulated within the hydrogels, which associated with a viability >80% for all cell types. These results demonstrated that fast gelation of silk fibroin can be achieved by combining it with riboflavin and electron acceptors, which results in a hydrogel that can be used in tissue engineering and cell delivery applications.

Graphical abstract: Rapid and cytocompatible cell-laden silk hydrogel formation via riboflavin-mediated crosslinking

Back to tab navigation

Supplementary files

Article information


Submitted
13 Jul 2020
Accepted
20 Sep 2020
First published
21 Sep 2020

This article is Open Access

J. Mater. Chem. B, 2020,8, 9566-9575
Article type
Paper

Rapid and cytocompatible cell-laden silk hydrogel formation via riboflavin-mediated crosslinking

S. Piluso, D. Flores Gomez, I. Dokter, L. Moreira Texeira, Y. Li, J. Leijten, R. van Weeren, T. Vermonden, M. Karperien and J. Malda, J. Mater. Chem. B, 2020, 8, 9566
DOI: 10.1039/D0TB01731K

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

Reproduced material should be attributed as follows:

  • For reproduction of material from NJC:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
  • For reproduction of material from PCCP:
    [Original citation] - Published by the PCCP Owner Societies.
  • For reproduction of material from PPS:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
  • For reproduction of material from all other RSC journals:
    [Original citation] - Published by The Royal Society of Chemistry.

Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.


Social activity

Search articles by author

Spotlight

Advertisements