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Issue 32, 2019
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A highly sensitive and selective two-photon fluorescent probe for glutathione S-transferase detection and imaging in living cells and tissues

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Abstract

Glutathione transferase (GST) is a very important metabolic enzyme that mediates the wide metabolism of endogenous and xenobiotic compounds; it usually has a significant over expression in cancer cells, which is a key reason resulting in drug resistance, and will show an obvious down regulation during liver injury, thus it was also regarded as a vital biomarker in clinical diagnosis. Herein, based on boron-dipyrromethene (BODIPY) dye, a two-photon probe BNPA was designed for the real-time detection of GST activities and fluorescence imaging in both cancer cells and liver tissues. Importantly, BNPA exhibited a high selectivity, ultrahigh imaging resolution and showed a classic Michaelis–Menten kinetics toward GSTs. Furthermore, it was successfully used for monitoring the GST activities in living cells and deep tissues by two-photon imaging, as well as detecting the down regulation of GST activities during α-naphthylisothiocyanate (ANIT) induced liver injury. Our results fully demonstrated that BNPA could serve as a promising tool for evaluating the GST function and the process of cellular GSTs in living systems, and also provided a new approach for studying GST-associated liver diseases, which would be greatly useful for rational drug use and disease diagnosis in clinics.

Graphical abstract: A highly sensitive and selective two-photon fluorescent probe for glutathione S-transferase detection and imaging in living cells and tissues

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Supplementary files

Article information


Submitted
28 Apr 2019
Accepted
11 Jul 2019
First published
16 Jul 2019

J. Mater. Chem. B, 2019,7, 4983-4989
Article type
Paper

A highly sensitive and selective two-photon fluorescent probe for glutathione S-transferase detection and imaging in living cells and tissues

Z. Tian, X. Tian, L. Feng, Y. Tian, X. Huo, B. Zhang, S. Deng, X. Ma and J. Cui, J. Mater. Chem. B, 2019, 7, 4983
DOI: 10.1039/C9TB00834A

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