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Issue 28, 2019
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NIR-excited superoxide radical procreators to eradicate tumors by targeting the lyso-membrane

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Abstract

NIR photosensitizers have been used as potential photo-activators to inhibit cancer cells as well as the growth of various types of bacteria. Herein, we first designed NIR alkylated cationic photosensitizers (Et-NB-C12 > Et-NB-C18 and Mt-NB-C12 > Mt-NB-C18), which target the lyso-membrane for the eradication of tumor cells through prominent superoxide radical generation (type-I PDT) via the lysosome disruption pathway. The O2˙ radical sensors (DHE and DHR123) exhibited a 10-fold increment in fluorescence intensity in solution and MCF-7 cells after treatment with NIR cationic photosensitizers under 660 nm irradiation. Et-NB-C12 > Et-NB-C18 preferentially targeted the lyso-membrane, resulting in O2˙ radical generation in the lyso-membranes during PDT irradiation. Furthermore, lysosomal disruption, flow cytometry, and MTT assays demonstrated that the influential generation of O2˙ radicals in cancer cells initiated cell apoptosis and further cell death after irradiation with PDT light at 660 nm. The inhibition activity of Et-NB-C12 > Et-NB-C18 in MCF-7 cells showed stronger anticancer efficiency (IC50 0.08 > 0.12 μM) than that of Mt-NB-C12 > Mt-NB-C18 (IC50 = 0.30 > 0.32 μM), lower than that of Ce6 (1.28 μM). In particular, Et-NB-C12 can be effectively utilized in in vivo imaging, and it exhibits outstanding anticancer properties during both ex vivo and in vivo photodynamic therapy.

Graphical abstract: NIR-excited superoxide radical procreators to eradicate tumors by targeting the lyso-membrane

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Publication details

The article was received on 15 Apr 2019, accepted on 10 Jun 2019 and first published on 14 Jun 2019


Article type: Paper
DOI: 10.1039/C9TB00721K
J. Mater. Chem. B, 2019,7, 4440-4450

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    NIR-excited superoxide radical procreators to eradicate tumors by targeting the lyso-membrane

    B. Gurram, M. Li, M. Li, K. H. Gebremedhin, W. Sun, J. Fan, J. Wang and X. Peng, J. Mater. Chem. B, 2019, 7, 4440
    DOI: 10.1039/C9TB00721K

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