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Issue 5, 2017
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Effective control of the intrinsic DNA morphology by photosensitive polyamines

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Abstract

Non-viral vectors for gene therapy such as DNA-cationic probe complexes offer important bio-safety advantages over viral approaches, due to their reduced pathogenicity, immunogenicity and cytotoxicity. In the present study we examine two polycationic water-soluble azobenzene derivatives (bis-Azo-2N and bis-Azo-3N) containing different linear unsubstituted polyamine moieties and we demonstrate the ability of such photochromes to destabilize the intrinsic B-DNA secondary structure in a concentration-dependent manner. Furthermore, through a detailed series of biophysical experiments, varying the photochrome conformation, temperature, salt and DNA concentration, we provide a detailed insight into the azobenzene–DNA binding pathway (Ka: bis-Azo-2N(trans)-DNA = 5.3 ± 0.3 × 104 M−1, Ka: bis-Azo-2N(cis)-DNA = 2.6 ± 0.2 × 104 M−1, Ka: bis-Azo-3N(trans)-DNA = 7.1 ± 0.4 × 104 M−1 and Ka: bis-Azo-3N(cis)-DNA = 5.1 ± 0.4 × 104 M−1) establishing the versatility of such materials as promising candidates for use in non-viral gene delivery systems.

Graphical abstract: Effective control of the intrinsic DNA morphology by photosensitive polyamines

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Supplementary files

Article information


Submitted
19 Oct 2016
Accepted
11 Dec 2016
First published
12 Dec 2016

This article is Open Access

J. Mater. Chem. B, 2017,5, 1028-1038
Article type
Paper

Effective control of the intrinsic DNA morphology by photosensitive polyamines

M. Deiana, Z. Pokladek, K. Matczyszyn, P. Mlynarz, M. Buckle and M. Samoc, J. Mater. Chem. B, 2017, 5, 1028 DOI: 10.1039/C6TB02732F

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