Ultrasensitive ELISA+ enhanced by dendritic mesoporous silica nanoparticles

Chang Lei; Chun Xu; Amanda Nouwens; Chengzhong Yu

doi:10.1039/C6TB01023G

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Ultrasensitive ELISA⁺ enhanced by dendritic mesoporous silica nanoparticles†

Chang Lei,^a Chun Xu,^a Amanda Nouwens^ab and Chengzhong Yu*^a

Author affiliations

* Corresponding authors

^a Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia
E-mail: c.yu@uq.edu.au

^b School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia

Abstract

An ultrasensitive enzyme-linked immunosorbent assay (ELISA⁺) has been developed by using dendritic mesoporous silica nanoparticles (DMSNs) with high horseradish peroxidase (HRP) loading and easily accessible pore channels for signal amplification. A 2000 times enhancement of detection sensitivity compared to a commercial ELISA kit for insulin detection in serum is achieved.

This article is part of the themed collection: 2016 Journal of Materials Chemistry B Hot Papers

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Article information

DOI: https://doi.org/10.1039/C6TB01023G
Article type: Communication
Submitted: 24 Apr 2016
Accepted: 27 Jun 2016
First published: 28 Jun 2016

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J. Mater. Chem. B, 2016,4, 4975-4979

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Ultrasensitive ELISA⁺ enhanced by dendritic mesoporous silica nanoparticles

C. Lei, C. Xu, A. Nouwens and C. Yu, J. Mater. Chem. B, 2016, 4, 4975 DOI: 10.1039/C6TB01023G

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Journal of Materials Chemistry B