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Issue 28, 2016
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Rapid fluorescence detection of hypoxic microenvironments by nitro-benzyl conjugated chitosan nanoparticles encapsulating hydrophobic fluorophores

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Abstract

In this study, hypoxia-responsive chitosan nanoparticles (HRCNs) were synthesized by the conjugation of nitro-benzyl derivatives into chitosan polymers and the subsequent self-assembly of them with hydrophobic fluorophores. The nitro-benzyl substrate of HRCNs could be selectively reduced by NTR/NADH that was overexpressed under hypoxic conditions, while it was not responsive to reductive agents (GSH or DTT) commonly existing in biological systems. The HRCN encapsulated with Rhodamine 6G (HRCN-R6G) was successfully applied for the rapid determination of the hypoxic status of lung carcinoma cells (A549) within 30 min, while several hours were required in a conventional assay. In the hypoxic microenvironment, the fluorescence intensity of HRCN-R6G was 2.6-fold higher than that in normoxic conditions. Taken together, the HRCN-R6G is expected to be used for the rapid diagnosis and treatment of hypoxia-related acute diseases, after the incorporation of therapeutic drugs into them.

Graphical abstract: Rapid fluorescence detection of hypoxic microenvironments by nitro-benzyl conjugated chitosan nanoparticles encapsulating hydrophobic fluorophores

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Supplementary files

Article information


Submitted
15 Apr 2016
Accepted
22 Jun 2016
First published
22 Jun 2016

J. Mater. Chem. B, 2016,4, 4832-4838
Article type
Paper

Rapid fluorescence detection of hypoxic microenvironments by nitro-benzyl conjugated chitosan nanoparticles encapsulating hydrophobic fluorophores

L. Ren, Y. J. Kim, S. Y. Park, S. Lee, J. Lee, C. P. Park and Y. T. Lim, J. Mater. Chem. B, 2016, 4, 4832
DOI: 10.1039/C6TB00935B

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