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Issue 6, 2015
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A three-dimensional amylopectin-reduced graphene oxide framework for efficient adsorption and removal of hemoglobin

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Abstract

A three-dimensional graphene oxide framework is prepared via a simple and cost-effective one-pot approach through the hydrogen-bonding interaction between amylopectin and graphene oxide in the presence of hydrazine hydrate acting as a reducing reagent. The framework is shortly termed as AP-rGO and it is characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), surface charge analysis and thermogravimetric analysis (TGA). The obtained AP-rGO framework exhibits excellent adsorption performance toward hemoglobin in the presence of other protein species. It provides a maximum adsorption capacity of 1010 mg g−1. In a neutral medium (at pH 7), 70 mg L−1 of Hb in 1.0 mL of aqueous solution could be effectively adsorbed by 1.0 mg of the AP-rGO framework, giving rise to an adsorption efficiency of 92.7%. The practical application of the AP-rGO framework is demonstrated by the removal of a highly abundant protein, i.e., hemoglobin, from complex biological sample matrices, e.g., human whole blood. The removal efficiency is well confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) assay.

Graphical abstract: A three-dimensional amylopectin-reduced graphene oxide framework for efficient adsorption and removal of hemoglobin

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Publication details

The article was received on 30 Oct 2014, accepted on 22 Dec 2014 and first published on 23 Dec 2014


Article type: Paper
DOI: 10.1039/C4TB01792G
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J. Mater. Chem. B, 2015,3, 983-989

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    A three-dimensional amylopectin-reduced graphene oxide framework for efficient adsorption and removal of hemoglobin

    Y. Zhang, J. Liu, X. Chen and J. Wang, J. Mater. Chem. B, 2015, 3, 983
    DOI: 10.1039/C4TB01792G

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