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Issue 48, 2014
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Site-specific immobilization of DNA on silicon surfaces by using the thiol–yne reaction

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Abstract

Covalent immobilization of ssDNA fragments onto silicon-based materials was performed using the thiol–yne reaction. Chemical functionalization provided alkyne groups on the surface where the thiol-modified oligonucleotide probes can be easily photoattached as microarrays, reaching an immobilization density around 30 pmol cm−2. The developed method presents the advantages of spatially controlled probe anchoring (by using a photomask), direct attachment without using cross-linkers, and short irradiation times (20 min). Hybridization efficiencies up to 70%, with full complementary strands, were reached. The approach was evaluated by scoring single nucleotide polymorphisms with a discrimination ratio around 15. Moreover, the potential applicability of the proposed methodology is demonstrated through the specific detection of 20 nM of a genomic target of bacterial Escherichia coli.

Graphical abstract: Site-specific immobilization of DNA on silicon surfaces by using the thiol–yne reaction

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The article was received on 07 Jul 2014, accepted on 01 Oct 2014 and first published on 01 Oct 2014


Article type: Paper
DOI: 10.1039/C4TB01108B
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Citation: J. Mater. Chem. B, 2014,2, 8510-8517

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    Site-specific immobilization of DNA on silicon surfaces by using the thiol–yne reaction

    J. Escorihuela, M. Bañuls, R. Puchades and Á. Maquieira, J. Mater. Chem. B, 2014, 2, 8510
    DOI: 10.1039/C4TB01108B

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