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Issue 31, 2014
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Thermoresponsive elastin/laminin mimicking artificial protein for modifying PLLA scaffolds in nerve regeneration

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Abstract

Poly(L-lactic acid) (PLLA) is widely used as a scaffold but does not possess biological functions. Here, we described the biosynthesis of the elastin-like repetitive polypeptide (VPGIG)30 containing a laminin-derived neurite outgrowth-promoting sequence (RKRLQVQLSIRT: AG73) (AG73-(VPGIG)30). The expression vector for AG73-(VPGIG)30 was constructed using the self-ligation technique to elongate the VPGIG repetitive sequence. The coacervation temperature of the purified AG73-(VPGIG)30 protein was 20 and 14 °C in water and phosphate-buffered saline (PBS), respectively. AG73-(VPGIG)30 was quickly adsorbed on PLLA films via a hydrophobic interaction by raising the temperature from 4 °C to 37 °C. On the AG73-(VPGIG)30-modified PLLA surface, the neurite outgrowth of PC12 cells was strongly promoted. We successfully induced the neurite outgrowth activity on PLLA films by treating the novel surface modifier AG73-(VPGIG)30, which could be applicable in developing PLLA scaffolds for nerve regeneration.

Graphical abstract: Thermoresponsive elastin/laminin mimicking artificial protein for modifying PLLA scaffolds in nerve regeneration

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Publication details

The article was received on 24 Feb 2014, accepted on 30 May 2014 and first published on 04 Jun 2014


Article type: Paper
DOI: 10.1039/C4TB00305E
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Citation: J. Mater. Chem. B, 2014,2, 5061-5067

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    Thermoresponsive elastin/laminin mimicking artificial protein for modifying PLLA scaffolds in nerve regeneration

    S. Kakinoki and T. Yamaoka, J. Mater. Chem. B, 2014, 2, 5061
    DOI: 10.1039/C4TB00305E

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