Jump to main content
Jump to site search

Issue 9, 2014
Previous Article Next Article

A polymer-based nanodevice for the photoregulated release of NO with two-photon fluorescence reporting in skin carcinoma cells

Author affiliations

Abstract

We have developed a multifunctional biocompatible nanoconstruct based on polymeric nanoparticles encapsulating a molecular conjugate, able to photorelease nitric oxide (NO) with a fluorescent reporting function. We demonstrate that two-photon excitation (TPE) using biofriendly NIR 700 nm laser light can be applied for monitoring as well as triggering the release of NO, wherein the uncaging of a strongly fluorescent co-product acts in turn as a TPE fluorescent reporter for the simultaneous NO release from the nanoassembly. This supramolecular nanodevice internalizes in skin carcinoma cells, induces significant cell death upon light excitation and preserves its TPE properties, allowing the nearly instantaneous quantification of the NO photoreleased in cancer cells by two-photon NIR fluorescence microscopy.

Graphical abstract: A polymer-based nanodevice for the photoregulated release of NO with two-photon fluorescence reporting in skin carcinoma cells

Back to tab navigation

Article information


Submitted
10 Oct 2013
Accepted
25 Nov 2013
First published
28 Nov 2013

J. Mater. Chem. B, 2014,2, 1190-1195
Article type
Paper

A polymer-based nanodevice for the photoregulated release of NO with two-photon fluorescence reporting in skin carcinoma cells

V. Kirejev, N. Kandoth, R. Gref, M. B. Ericson and S. Sortino, J. Mater. Chem. B, 2014, 2, 1190
DOI: 10.1039/C3TB21414A

Social activity

Search articles by author

Spotlight

Advertisements