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Issue 7, 2014
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Large pore raspberry textured phosphonate@silica nanoparticles for protein immobilization

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Abstract

This paper reports the synthesis of large pore (11 nm) monodisperse raspberry textured phosphonate@silica nanoparticles (70–90 nm) with high capacity for protein immobilization. The raspberry nanoparticles denoted RNP_PME(2.5) with phosphonate loading 2.5 mmol g−1, formed using an organosilanephosphonate (MeO)3SiCH2CH2PO(OMe)2, as silica surface modifier and structure directing agent. Specific reaction conditions including temperature and concentration of phosphonate, base, surfactant and co-solvent were required for RNP_PME(2.5) formation. Rhodamine B labelled RNP_PME(2.5) was readily internalised by HeLa cells with no deficit of cell viability. Aqueous dispersions of RNP_PME(2.5) were stable over several months. In protein immobilization studies using BSA, bovine serum albumin, with RNP_PME(2.5), smaller pore (∼3 nm) phosphonate@silica nanoparticles NP_PME(1.0) and NP_PME(0.2) and mesoporous silica nanoparticles, MSN, the large pore RNP_PME(2.5) gave highest BSA loading 266 mg g−1, formed the most stable aqueous dispersions (BSA@MSN was unstable and precipitated) and gave the best protection against BSA structural distortion at pH 7.4.

Graphical abstract: Large pore raspberry textured phosphonate@silica nanoparticles for protein immobilization

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Supplementary files

Article information


Submitted
13 Sep 2013
Accepted
16 Dec 2013
First published
24 Dec 2013

J. Mater. Chem. B, 2014,2, 903-914
Article type
Paper

Large pore raspberry textured phosphonate@silica nanoparticles for protein immobilization

S. P. Maddala, D. Velluto, Z. Luklinska and A. C. Sullivan, J. Mater. Chem. B, 2014, 2, 903
DOI: 10.1039/C3TB21263G

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