Jump to main content
Jump to site search

Issue 5, 2014
Previous Article Next Article

Endothelial cell thrombogenicity is reduced by ATRP-mediated grafting of gelatin onto PCL surfaces

Author affiliations

Abstract

Reducing the thrombogenicity of a tissue-engineered vascular graft prior to implantation is important for improving graft patency. As functionalization of synthetic materials with cell-adhesive proteins is routinely utilized as a means to promote endothelial cell (EC) growth, we conducted detailed investigation on the proliferation and thrombogenicity of ECs on such functionalized surfaces. We observed that polycaprolactone (PCL) surfaces functionalized with poly(glycidyl methacrylate) [(P(GMA)] brushes via atom transfer radical polymerization (ATRP) alone resulted in the enhancement of an activated EC profile characterized by low production of nitric oxide (NO), platelet activation and elevated expression levels of von Willebrand factor (vWF) and matrix metalloproteinase-2 (MMP-2). When gelatin was conjugated onto the PCL-g-P(GMA) surfaces, not only were EC proliferation and endothelial coverage significantly improved, but an anti-thrombogenic profile was also observed. We demonstrated that PCL can be successfully functionalized by a controllable surface-initiated polymerization method and importantly, the thrombogenic profile of the endothelial cells can be influenced by material surface chemistry (e.g. the presence of polymer graft chains). Our findings emphasize the importance of a careful consideration of materials for vascular graft applications, as well as differential endothelial cell physiology on surfaces with different material chemistry.

Graphical abstract: Endothelial cell thrombogenicity is reduced by ATRP-mediated grafting of gelatin onto PCL surfaces

Back to tab navigation

Supplementary files

Publication details

The article was received on 29 May 2013, accepted on 11 Nov 2013 and first published on 11 Nov 2013


Article type: Paper
DOI: 10.1039/C3TB20760A
J. Mater. Chem. B, 2014,2, 485-493

  •   Request permissions

    Endothelial cell thrombogenicity is reduced by ATRP-mediated grafting of gelatin onto PCL surfaces

    G. M. Xiong, S. Yuan, C. K. Tan, J. K. Wang, Y. Liu, T. T. Yang Tan, N. S. Tan and C. Choong, J. Mater. Chem. B, 2014, 2, 485
    DOI: 10.1039/C3TB20760A

Search articles by author

Spotlight

Advertisements