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Issue 1, 2013
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Combining magnetic field/temperature dual stimuli to significantly enhance gene transfection of nonviral vectors

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Abstract

Monodisperse magnetic nanoparticles (MNPs) were prepared through an organic phase process, and the obtained MNPs were capped with poly[2-(2-methoxyethoxy)ethyl methacrylate]-b-poly[2-(dimethylamino)ethyl methacrylate] synthesized by surface-initiated atom transfer radical polymerization (ATRP). The MNPs-polymer brushes exhibited both superparamagnetic and thermoresponsive behaviors, and could condense plasmid DNA into nanocomplexes with a size of 100–120 nm at appropriate complexing ratios. Enhanced gene expression in COS-7 cells and HepG-2 cells was achieved under a magnetic field and variable temperature conditions due to magnetic force-facilitated internalization of nanocomplexes, and temporary cooling-triggered intracellular gene unpacking. Amazingly, combining magnetic field and temperature dual stimuli contributed to a 50–100- and 25–45-fold increase of the transfection efficiency in HepG-2 cells compared to conventional protocol and PEI25k, respectively.

Graphical abstract: Combining magnetic field/temperature dual stimuli to significantly enhance gene transfection of nonviral vectors

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Supplementary files

Article information


Submitted
03 Oct 2012
Accepted
24 Oct 2012
First published
24 Oct 2012

J. Mater. Chem. B, 2013,1, 43-51
Article type
Paper

Combining magnetic field/temperature dual stimuli to significantly enhance gene transfection of nonviral vectors

H. Wang, J. Yang, Y. Li, L. Sun and W. Liu, J. Mater. Chem. B, 2013, 1, 43
DOI: 10.1039/C2TB00203E

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