Jump to main content
Jump to site search
Publishing
Advanced

Issue 1, 2013
Previous Article Next Article

Cellular uptake and gene delivery using layered double hydroxide nanoparticles†

Shuangde Li,ac   Jinghuan Li,b   Chengle J. Wang,c   Qiang Wang,c   M. Zameel Cader,b   Jun Lu,*a   David G. Evans,a   Xue Duana  and  Dermot O'Hare*c  
Author affiliations

* Corresponding authors

a State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, P. R. China
E-mail: lujun@mail.buct.edu.cn
Fax: +86-10-64425385
Tel: +86-10-64412131

b Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK

c Chemistry Research Laboratory, Department of Chemistry, University of Oxford, Mansfield Road, Oxford OX1 3TA, UK
E-mail: dermot.ohare@chem.ox.ac.uk
Tel: +44 (0)1865 272686

Abstract

The cellular uptake of narrowly dispersed LDH {[Mg3Al(OH)8](CO3)0.5} nanoparticles into the Mouse Motor Neuron (NSC 34) cell line has been studied. The effect of LDH concentration and incubation time on the cellular uptake was investigated using fluorescein isothiocyanate (FITC) labelled LDH nanoparticles. We observed that cellular uptake increases with the increased LDHs concentration and incubation time. Confocal laser microscopy and transmission electron microscopy reveal that 20 nm LDHs nanoparticles intrude into the cytoplasm and then enrich in the cellular nucleus, while nanoparticles greater than 20 nm only locate in the cytoplasm. The 20 nm sized LDHs nanoparticles display similar uptake to both the cytoplasm and nucleus, and show little cytotoxicity with no significant decrease in NSC 34 cell proliferation and viability below 200 μg ml−1. DNA modified 20 nm LDH nanoparticles are successful in transfection of the pEGFP-N1 DNA plasmid to NSC 34 cells.

Graphical abstract: Cellular uptake and gene delivery using layered double hydroxide nanoparticles

Back to tab navigation
Download options Please wait...

Supplementary files

Article information

https://doi.org/10.1039/C2TB00081D
Submitted
05 Sep 2012
Accepted
17 Sep 2012
First published
16 Oct 2012
J. Mater. Chem. B, 2013,1, 61-68
Article type
Paper
Permissions
Request permissions

Cellular uptake and gene delivery using layered double hydroxide nanoparticles

S. Li, J. Li, C. J. Wang, Q. Wang, M. Z. Cader, J. Lu, D. G. Evans, X. Duan and D. O'Hare, J. Mater. Chem. B, 2013, 1, 61
DOI: 10.1039/C2TB00081D

If you are not the author of this article and you wish to reproduce material from it in a third party non-RSC publication you must formally request permission using Copyright Clearance Center. Go to our Instructions for using Copyright Clearance Center page for details.

Authors contributing to RSC publications (journal articles, books or book chapters) do not need to formally request permission to reproduce material contained in this article provided that the correct acknowledgement is given with the reproduced material.

Reproduced material should be attributed as follows:

  • For reproduction of material from NJC:
    Reproduced from Ref. XX with permission from the Centre National de la Recherche Scientifique (CNRS) and The Royal Society of Chemistry.
  • For reproduction of material from PCCP:
    Reproduced from Ref. XX with permission from the PCCP Owner Societies.
  • For reproduction of material from PPS:
    Reproduced from Ref. XX with permission from the European Society for Photobiology, the European Photochemistry Association, and The Royal Society of Chemistry.
  • For reproduction of material from all other RSC journals and books:
    Reproduced from Ref. XX with permission from The Royal Society of Chemistry.

If the material has been adapted instead of reproduced from the original RSC publication "Reproduced from" can be substituted with "Adapted from".

In all cases the Ref. XX is the XXth reference in the list of references.

If you are the author of this article you do not need to formally request permission to reproduce figures, diagrams etc. contained in this article in third party publications or in a thesis or dissertation provided that the correct acknowledgement is given with the reproduced material.

Reproduced material should be attributed as follows:

  • For reproduction of material from NJC:
    [Original citation] - Reproduced by permission of The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC
  • For reproduction of material from PCCP:
    [Original citation] - Reproduced by permission of the PCCP Owner Societies
  • For reproduction of material from PPS:
    [Original citation] - Reproduced by permission of The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC
  • For reproduction of material from all other RSC journals:
    [Original citation] - Reproduced by permission of The Royal Society of Chemistry

If you are the author of this article you still need to obtain permission to reproduce the whole article in a third party publication with the exception of reproduction of the whole article in a thesis or dissertation.

Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Social activity

Search articles by author

Spotlight

Advertisements