Structure and dynamics of lipid membranes interacting with antivirulence end-phosphorylated polyethylene glycol block copolymers

Jing Yu; Jun Mao; Michihiro Nagao; Wei Bu; Binhua Lin; Kunlun Hong; Zhang Jiang; Yun Liu; Shuo Qian; Matthew Tirrell; Wei Chen

doi:10.1039/C9SM01642B

Structure and dynamics of lipid membranes interacting with antivirulence end-phosphorylated polyethylene glycol block copolymers†‡

Jing Yu,

§^ab Jun Mao,^ab Michihiro Nagao,

^cd Wei Bu,

^e Binhua Lin,

^ef Kunlun Hong,

^g Zhang Jiang,^h Yun Liu,^ci Shuo Qian,

^j Matthew Tirrell

*^ab and Wei Chen

*^ab

Author affiliations

* Corresponding authors

^a Centre for Molecular Engineering and Materials Science Division, Argonne National Laboratory, Lemont, IL, USA
E-mail: wchen@anl.gov

^b Pritzker School of Molecular Engineering, University of Chicago, Chicago, IL, USA
E-mail: mtirrell@uchicago.edu

^c NIST Centre for Neutron Research, National Institute of Standards and Technology, Gaithersburg, MD, USA

^d Centre for Exploration of Energy and Matter, Indiana University, Bloomington, IN, USA

^e Centre for Advanced Radiation Sources, University of Chicago, Chicago, IL, USA

^f James Franck Institute, University of Chicago, Chicago, IL, USA

^g Centre for Nanophase Materials Sciences, Oak Ridge National Laboratory, Oak Ridge, TN, USA

^h Advanced Photon Source, Argonne National Laboratory, Lemont, IL, USA

ⁱ Department of Chemical and Biomolecular Engineering, Centre for Neutron Science, University of Delaware, Newark, DE, USA

^j Biology and Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA

Abstract

The structure and dynamics of lipid membranes in the presence of extracellular macromolecules are critical for cell membrane functions and many pharmaceutical applications. The pathogen virulence-suppressing end-phosphorylated polyethylene glycol (PEG) triblock copolymer (Pi-ABAPEG) markedly changes the interactions with lipid vesicle membranes and prevents PEG-induced vesicle phase separation in contrast to the unphosphorylated copolymer (ABAPEG). Pi-ABAPEG weakly absorbs on the surface of lipid vesicle membranes and slightly changes the structure of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) unilamellar vesicles at 37 °C, as evidenced by small angle neutron scattering. X-ray reflectivity measurements confirm the weak adsorption of Pi-ABAPEG on DMPC monolayer, resulting in a more compact DMPC monolayer structure. Neutron spin-echo results show that the adsorption of Pi-ABAPEG on DMPC vesicle membranes increases the membrane bending modulus κ.

Soft Matter

Structure and dynamics of lipid membranes interacting with antivirulence end-phosphorylated polyethylene glycol block copolymers†‡

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Structure and dynamics of lipid membranes interacting with antivirulence end-phosphorylated polyethylene glycol block copolymers

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