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Issue 6, 2019
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Colloid supported lipid bilayers for self-assembly

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Abstract

The use of colloid supported lipid bilayers (CSLBs) has recently been extended to create colloidal joints, that enable the assembly of structures with internal degrees of flexibility, and to study lipid membranes on curved and closed geometries. These novel applications of CSLBs rely on previously unappreciated properties: the simultaneous fluidity of the bilayer, lateral mobility of inserted (linker) molecules and colloidal stability. Here we characterize every step in the manufacturing of CSLBs in view of these requirements using confocal microscopy and fluorescence recovery after photobleaching (FRAP). Specifically, we have studied the influence of different particle properties (roughness, surface charge, chemical composition, polymer coating) on the quality and mobility of the supported bilayer. We find that the insertion of lipopolymers in the bilayer can affect its homogeneity and fluidity. We improve the colloidal stability by inserting lipopolymers or double-stranded inert DNA into the bilayer. We include surface-mobile DNA linkers and use FRAP to characterize their lateral mobility both in their freely diffusive and bonded state. Finally, we demonstrate the self-assembly of flexibly linked structures from the CSLBs modified with surface-mobile DNA linkers. Our work offers a collection of experimental tools for working with CSLBs in applications ranging from controlled bottom-up self-assembly to model membrane studies.

Graphical abstract: Colloid supported lipid bilayers for self-assembly

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Publication details

The article was received on 13 Aug 2018, accepted on 23 Oct 2018 and first published on 19 Dec 2018


Article type: Paper
DOI: 10.1039/C8SM01661E
Citation: Soft Matter, 2019,15, 1345-1360
  • Open access: Creative Commons BY-NC license
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    Colloid supported lipid bilayers for self-assembly

    M. Rinaldin, R. W. Verweij, I. Chakraborty and D. J. Kraft, Soft Matter, 2019, 15, 1345
    DOI: 10.1039/C8SM01661E

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