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Issue 48, 2014
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Coiled coil driven membrane fusion between cyclodextrin vesicles and liposomes

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Abstract

Controlled fusion events between natural membranes composed of phospholipids with synthetic unnatural membranes will yield valuable fundamental information on the mechanism of membrane fusion. Here, fusion between vastly different phospholipid liposomes and cyclodextrin amphiphile based vesicles (CDVs) controlled by a pair of coiled coil forming lipidated peptides was investigated. Fusion events were characterized using lipid and content mixing assays and the resulting hybrid assemblies were characterized with cryo-TEM imaging. The secondary/quaternary structure of the lipidated peptides at the membrane interface was studied using circular dichroism spectroscopy. This is the first example of targeted fusion between natural and non-natural bilayer membranes and the in situ formation of hybrid CDV–liposome structures is of interest as it yields fundamental information about the mechanism through which fusion proceeds.

Graphical abstract: Coiled coil driven membrane fusion between cyclodextrin vesicles and liposomes

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Supplementary files

Article information


Submitted
14 Aug 2014
Accepted
27 Oct 2014
First published
28 Oct 2014

Soft Matter, 2014,10, 9746-9751
Article type
Paper

Coiled coil driven membrane fusion between cyclodextrin vesicles and liposomes

F. Versluis, J. Voskuhl, J. Vos, H. Friedrich, B. J. Ravoo, P. H. H. Bomans, M. C. A. Stuart, N. A. J. M. Sommerdijk and A. Kros, Soft Matter, 2014, 10, 9746
DOI: 10.1039/C4SM01801J

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