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Issue 41, 2014
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Thin multicomponent films for functional enzyme devices and bioreactor particles

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Abstract

Complex functional films containing enzymes and other biomolecules are easily fabricated in nm-scale thicknesses by using layer-by-layer (LbL) methodologies first popularized by Lvov and Decher. In this review, we highlight the high level functional capabilities possible with LbL films of biomolecules based on our own research experiences. We first describe the basics of enzyme film fabrication by LbL alternate electrostatic adsorption, then discuss how to make functional enzyme–polyion films of remarkably high stability. Focusing on cytochrome P450s, we discuss films developed to electrochemically activate the natural catalytic cycle of these key metabolic enzymes. We then describe multifunctional, multicomponent DNA/enzyme/polyion films on arrays and particle surfaces for high throughput metabolic toxicity screening using electrochemiluminescence and LC-MS/MS. Using multicomponent LbL films, complex functionality for bioanalytical and biochemical purposes can be achieved that is difficult or impossible using conventional approaches.

Graphical abstract: Thin multicomponent films for functional enzyme devices and bioreactor particles

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Article information


Submitted
29 Jul 2014
Accepted
22 Aug 2014
First published
22 Aug 2014

Soft Matter, 2014,10, 8145-8156
Article type
Tutorial Review
Author version available

Thin multicomponent films for functional enzyme devices and bioreactor particles

J. F. Rusling, D. P. Wasalathanthri and J. B. Schenkman, Soft Matter, 2014, 10, 8145
DOI: 10.1039/C4SM01679C

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