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Issue 14, 2014
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Intracellular stresses in patterned cell assemblies

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Abstract

Confining cells on adhesive patterns allows performing robust, weakly dispersed, statistical analysis. A priori, adhesive patterns could be efficient tools to analyze intracellular cell stress fields, in particular when patterns are used to force the geometry of the cytoskeleton. This tool could then be very helpful in deciphering the relationship between the internal architecture of the cells and the mechanical, intracellular stresses. However, the quantification of the intracellular stresses is still something delicate to perform. Here we first propose a new, very simple and original method to quantify the intracellular stresses, which directly relates the strain the cells impose on the extracellular matrix to the intracellular stress field. This method is used to analyze how confinement influences the intracellular stress field. As a result, we show that the more confined the cells are, the more stressed they will be. The influence of the geometry of the adhesive patterns on the stress patterns is also discussed.

Graphical abstract: Intracellular stresses in patterned cell assemblies

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Submitted
02 Sep 2013
Accepted
12 Dec 2013
First published
17 Dec 2013

Soft Matter, 2014,10, 2414-2423
Article type
Paper

Intracellular stresses in patterned cell assemblies

M. Moussus, C. D. Loughian, D. Fuard, M. Courçon, D. Gulino-Debrac, H. Delanoë-Ayari and A. Nicolas, Soft Matter, 2014, 10, 2414
DOI: 10.1039/C3SM52318G

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