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Issue 27, 2012
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Exploring thermal reversible hydrogels for stem cell expansion in three-dimensions

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Abstract

In this study, we report the application of a biocompatible thermo-reversible hydrogel, made from thermo-sensitive poly(N-isopropylacrylamide-co-acrylic acid) (P(NIPAM-AA)) microgels, for expanding stem cells in three-dimensions (3-D). The P(NIPAM-AA) microgels were synthesized by emulsion polymerization with their thermo-responsive phase transition behaviors being examined by light scattering and rheological methods. The viability of the microgel-exposed C3H/10T1/2 cells compared to the control cells is close to 100%, indicating the non-cytotoxicity of the synthesized microgels. At 37 °C, rheological measurements reveal the formation of hydrogels from 30 mg mL−1 microgel dispersions. The cross-sectional morphologies of the hydrogels show the interconnected porous structure. The 3-D stem cell culture system can be achieved by heating the microgel and cell mixtures to 37 °C. The increase of the viable stem cells cultured suggests that the in situ formed hydrogels support stem cell proliferation. The recovery of the 3-D cultured stem cells can be easily accomplished by cooling the culture system to room temperature. The released 3-D cultured cells can further adhere to a 2-D substrate, implying that the cultured stem cells are not only alive, but also retain the capability of migration. Therefore, the in situ formed thermo-reversible P(NIPAM-AA) hydrogels can be employed to expand stem cells in 3-D for further applications in tissue engineering.

Graphical abstract: Exploring thermal reversible hydrogels for stem cell expansion in three-dimensions

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Supplementary files

Article information


Submitted
22 Feb 2012
Accepted
13 May 2012
First published
11 Jun 2012

Soft Matter, 2012,8, 7250-7257
Article type
Paper

Exploring thermal reversible hydrogels for stem cell expansion in three-dimensions

Z. Shen, J. Bi, B. Shi, D. Nguyen, C. J. Xian, H. Zhang and S. Dai, Soft Matter, 2012, 8, 7250
DOI: 10.1039/C2SM25407G

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