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Issue 34, 2012
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Fluorescence correlation spectroscopy and Brownian dynamics simulation of protein diffusion under confinement in lipid cubic phases

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Abstract

The diffusion coefficient of protein lysozyme confined in lipid monoolein cubic phases was measured by fluorescence correlation spectroscopy. From the ratio of the diffusion coefficient of confined lysozyme to the one in a bulk solution, the barrier of confined diffusion was estimated to be about 3–4kBT at 20 °C. The origin of the barrier was investigated using Brownian dynamics simulations with model cubic phase structures based on so-called infinite periodic minimal surfaces. The simulations suggested a structure of the confinement as well as the importance of the hydrodynamic interaction between a particle and walls.

Graphical abstract: Fluorescence correlation spectroscopy and Brownian dynamics simulation of protein diffusion under confinement in lipid cubic phases

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Supplementary files

Article information


Submitted
06 Jan 2012
Accepted
26 Jun 2012
First published
23 Jul 2012

Soft Matter, 2012,8, 8936-8943
Article type
Paper

Fluorescence correlation spectroscopy and Brownian dynamics simulation of protein diffusion under confinement in lipid cubic phases

S. Tanaka, Soft Matter, 2012, 8, 8936
DOI: 10.1039/C2SM00045H

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