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Issue 20, 2012
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Composition and structure of mixed phospholipid supported bilayers formed by POPC and DPPC

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Abstract

In this paper we present a systematic study of the morphology and composition of supported lipid bilayers (SLBs) formed by vesicle fusion using a wide variety of surface sensitive techniques that give information about the lateral as well as vertical structure and bilayer fluidity. SLBs of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) mixtures at five different bulk vesicle compositions were formed in such a way that the phase separation boundaries were crossed. For all compositions studied, the SLBs were systematically enriched with POPC compared to the nominal vesicle composition. Nevertheless, gel-fluid domain coexistence was observed for SLB compositions in which phase separation was expected based on the bulk phase diagram. The probable causes for the compositional difference in the SLBs are discussed in terms of the phase behaviour of the mixture and its effect on the membrane formation process by vesicle fusion.

Graphical abstract: Composition and structure of mixed phospholipid supported bilayers formed by POPC and DPPC

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Supplementary files

Article information


Submitted
03 Jan 2012
Accepted
09 Mar 2012
First published
17 Apr 2012

Soft Matter, 2012,8, 5658-5665
Article type
Paper

Composition and structure of mixed phospholipid supported bilayers formed by POPC and DPPC

A. Åkesson, T. Lind, N. Ehrlich, D. Stamou, H. Wacklin and M. Cárdenas, Soft Matter, 2012, 8, 5658
DOI: 10.1039/C2SM00013J

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