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Issue 21, 2011
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Dual Click reactions to micropattern proteins

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In the study described in this report orthogonal Click reactions were utilized to immobilize two different proteins on surfaces side-by-side and in multilayer constructs. Alkyne- and azide-functionalized poly(ethylene glycol) hydrogel features were fabricated. Copper-catalyzed Huisgen 1,3 dipolar cycloaddition and oxime chemistry were employed to conjugate an azide-functionalized ubiquitin and oxoamide-modified myoglobin, respectively. Multicomponent patterning was verified by fluorescence imaging.

Graphical abstract: Dual Click reactions to micropattern proteins

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The article was received on 27 May 2011, accepted on 09 Aug 2011 and first published on 30 Aug 2011

Article type: Paper
DOI: 10.1039/C1SM05991B
Soft Matter, 2011,7, 9972-9977

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    Dual Click reactions to micropattern proteins

    R. M. Broyer, E. Schopf, C. M. Kolodziej, Y. Chen and H. D. Maynard, Soft Matter, 2011, 7, 9972
    DOI: 10.1039/C1SM05991B

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