Jump to main content
Jump to site search

Issue 20, 2010
Previous Article Next Article

Surface-modified hyaluronic acid hydrogels to capture endothelial progenitor cells

Author affiliations

Abstract

A major challenge to the effective treatment of injured cardiovascular tissues is the promotion of endothelialization of damaged tissues and implanted devices. For this reason, there is a need for new biomaterials that promote endothelialization to enhance vascular repair. The goal of this work was to develop antibody-modified polysaccharide-based hydrogels that could selectively capture endothelial progenitor cells (EPCs). We showed that CD34 antibody immobilization on hyaluronic acid (HA) hydrogels provides a suitable surface to capture EPCs. The effect of CD34 antibody immobilization on EPC adhesion was found to be dependent on antibody concentration. The highest level of EPC attachment was found to be 52.2 cells per mm2 on 1% HA gels modified with 25 μg mL−1 antibody concentration. Macrophages did not exhibit significant attachment on these modified hydrogel surfaces compared to the EPCs, demonstrating the selectivity of the system. Hydrogels containing only HA, with or without immobilized CD34, did not allow for spreading of EPCs 48 h after cell seeding, even though the cells were adhered to the hydrogel surface. To promote spreading of EPCs, 2% (w/v) gelatin methacrylate (GelMA) containing HA hydrogels were synthesized and shown to improve cell spreading and elongation. This strategy could potentially be useful to enhance the biocompatibility of implants such as artificial heart valves or in other tissue engineering applications where formation of vascular structures is required.

Graphical abstract: Surface-modified hyaluronic acid hydrogels to capture endothelial progenitor cells

Back to tab navigation

Supplementary files

Publication details

The article was received on 11 Jun 2010, accepted on 15 Jul 2010 and first published on 23 Aug 2010


Article type: Paper
DOI: 10.1039/C0SM00508H
Soft Matter, 2010,6, 5120-5126

  •   Request permissions

    Surface-modified hyaluronic acid hydrogels to capture endothelial progenitor cells

    G. Camci-Unal, H. Aubin, A. F. Ahari, H. Bae, J. W. Nichol and A. Khademhosseini*, Soft Matter, 2010, 6, 5120
    DOI: 10.1039/C0SM00508H

Search articles by author

Spotlight

Advertisements