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Issue 23, 2010
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Solubility limits of cholesterol, lanosterol, ergosterol, stigmasterol, and β-sitosterol in electroformed lipid vesicles

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Abstract

Here we use nuclear magnetic resonance to measure the solubility limit of several biologically relevant sterols in electroformed giant unilamellar vesicle membranes containing phosphatidylcholine (PC) lipids in ratios of 1 : 1 : X of DOPC : DPPC : sterol. We find solubility limits of cholesterol, lanosterol, ergosterol, stigmasterol, and β-sitosterol to be 65–70 mol%, ∼35 mol%, 30–35 mol%, 20–25 mol%, and ∼40 mol%, respectively. The low solubilities of stigmasterol and β-sitosterol, which differ from cholesterol only in their alkyl tails, show that subtle differences in tail structure can strongly affect sterol solubility. Below the solubility limits, the fraction of sterol to PC-lipid in electroformed vesicles linearly reflects the fraction in the original stock solutions used in the electroformation process.

Graphical abstract: Solubility limits of cholesterol, lanosterol, ergosterol, stigmasterol, and β-sitosterol in electroformed lipid vesicles

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Publication details

The article was received on 12 May 2010, accepted on 30 Jun 2010 and first published on 11 Aug 2010


Article type: Paper
DOI: 10.1039/C0SM00373E
Citation: Soft Matter, 2010,6, 5882-5890

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    Solubility limits of cholesterol, lanosterol, ergosterol, stigmasterol, and β-sitosterol in electroformed lipid vesicles

    M. M. Stevens, A. R. Honerkamp-Smith and S. L. Keller, Soft Matter, 2010, 6, 5882
    DOI: 10.1039/C0SM00373E

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