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Issue 21, 2010
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A modulatory bifunctional artificial enzyme with both SOD and GPx activities based on a smart star-shaped pseudo-block copolymer

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Abstract

An artificial smart bifunctional enzyme with both superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities was successfully constructed by the self-assembly of a porphyrin core with four suspensory adamantyl moieties and β-cyclodextrin-terminated temperature-sensitive copolymer through host–guest interaction in aqueous solution. The Mn(III) porphyrin, Mn(III)meso-tetra[1-(1-adamantyl methyl ketone)-4-pyridyl] porphyrin (MnTPyP-M-Ad), was designed as both a “supramolecular linker” and an efficient active site of SOD and the temperature-responsive block copolymer (β-CD-PEG-b-PNIPAAm-Te) with incorporated tellurium moieties in the PNIPAAm chain as GPx active sites was synthesized by ATRP. As a new type of artificial bifunctional enzymes, it exhibited stable SOD-like activity of 0.137 μM (IC50) at 37 °C, and high GPx catalytic efficiency with temperature-responsive dependence characteristic, and the new artificial enzyme was found to exhibit the highest GPx catalytic efficiency (v0 = 8.11 μM min−1) in close to physical temperature.

Graphical abstract: A modulatory bifunctional artificial enzyme with both SOD and GPx activities based on a smart star-shaped pseudo-block copolymer

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Supplementary files

Article information


Submitted
26 Mar 2010
Accepted
27 Jun 2010
First published
25 Aug 2010

Soft Matter, 2010,6, 5342-5350
Article type
Paper

A modulatory bifunctional artificial enzyme with both SOD and GPx activities based on a smart star-shaped pseudo-block copolymer

S. Yu, Y. Yin, J. Zhu, X. Huang, Q. Luo, J. Xu, J. Shen and J. Liu, Soft Matter, 2010, 6, 5342
DOI: 10.1039/C0SM00162G

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