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Issue 21, 2010
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Enzyme switch by complementary polymer pair system (CPPS)

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Manipulation of enzyme activity at will presents various research and industrial applications. Herein we describe development of a technology for inactivation and reactivation of enzyme activities using a polyanionic poly(acrylic acid) (PAAc) and a polycationic poly(allylamine) (PAA). Enzyme activities of ribonuclease A (RNase A), lysozyme, cellulase, and α-amylase were lost through addition of PAAc or PAA because of their binding to the enzymes. The activity of these enzymes except for α-amylase was then fully restored from the complex by the addition of oppositely charged polymers. Such manipulation of enzyme activity using a complementary polymer pair system (CPPS) enables the expansion of biomedical and biotechnological applications of the enzymes, including realization of protein delivery and intelligent bioreactors.

Graphical abstract: Enzyme switch by complementary polymer pair system (CPPS)

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Article information

15 Jan 2010
13 May 2010
First published
01 Jul 2010

Soft Matter, 2010,6, 5320-5326
Article type

Enzyme switch by complementary polymer pair system (CPPS)

S. Tomita, L. Ito, H. Yamaguchi, G. Konishi, Y. Nagasaki and K. Shiraki, Soft Matter, 2010, 6, 5320
DOI: 10.1039/C000930J

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