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Issue 21, 2010
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Enzyme switch by complementary polymer pair system (CPPS)

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Abstract

Manipulation of enzyme activity at will presents various research and industrial applications. Herein we describe development of a technology for inactivation and reactivation of enzyme activities using a polyanionic poly(acrylic acid) (PAAc) and a polycationic poly(allylamine) (PAA). Enzyme activities of ribonuclease A (RNase A), lysozyme, cellulase, and α-amylase were lost through addition of PAAc or PAA because of their binding to the enzymes. The activity of these enzymes except for α-amylase was then fully restored from the complex by the addition of oppositely charged polymers. Such manipulation of enzyme activity using a complementary polymer pair system (CPPS) enables the expansion of biomedical and biotechnological applications of the enzymes, including realization of protein delivery and intelligent bioreactors.

Graphical abstract: Enzyme switch by complementary polymer pair system (CPPS)

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Article information


Submitted
15 Jan 2010
Accepted
13 May 2010
First published
01 Jul 2010

Soft Matter, 2010,6, 5320-5326
Article type
Paper

Enzyme switch by complementary polymer pair system (CPPS)

S. Tomita, L. Ito, H. Yamaguchi, G. Konishi, Y. Nagasaki and K. Shiraki, Soft Matter, 2010, 6, 5320
DOI: 10.1039/C000930J

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