Issue 11, 2009
From the journal:

Soft Matter

Temperature-controlled positioning of fusion proteins in microreactors†

Rosalie L. M. Teeuwen,ab   Han Zuilhof,*b   Frits A. de Wolf*c  and  Jan C. M. van Hest*a  

* Corresponding authors

a Radboud University Nijmegen, Institute for Molecules and Materials, Department of Organic Chemistry, P.O. Box 9010, Nijmegen, GL, The Netherlands

b Wageningen UR, Laboratory for Organic Chemistry, P.O. Box 8026, Wageningen, EG, The Netherlands

c Wageningen UR, Agrotechnology & Food Innovations B.V., P.O. Box 17, Wageningen, AA, The Netherlands

Abstract

We present a non-covalent immobilization system based on stimulus-responsive elastin-like polypeptides (ELPs) to facilitate the positioning of proteins in microchannels. Two ELP variants were constructed and connected to fluorescent proteins EGFP and DsRed2. These fusion proteins display an inverse transition behavior that can be simply controlled by varying concentrations of NaCl. With these ELP fusion proteins two patches of fluorescent proteins can be formed inside a microreactor, using only the temperature-responsive property of ELPs.

Graphical abstract: Temperature-controlled positioning of fusion proteins in microreactors

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Article information

DOI
https://doi.org/10.1039/B903087E
Article type
Paper
Submitted
13 Feb 2009
Accepted
27 Mar 2009
First published
06 May 2009

Soft Matter, 2009,5, 2261-2268

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Temperature-controlled positioning of fusion proteins in microreactors

R. L. M. Teeuwen, H. Zuilhof, F. A. de Wolf and J. C. M. van Hest, Soft Matter, 2009, 5, 2261 DOI: 10.1039/B903087E

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