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Issue 11, 2008
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Lipid layers on polyelectrolyte multilayer supports

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Abstract

The mechanism of formation of supported lipid layers from phosphatidylcholine and phosphatidylserine vesicles in solution on polyelectrolyte multilayers was studied by a variety of experimental techniques. The interaction of zwitterionic and acidic lipid vesicles, as well as their mixtures, with polyelectrolyte supports was followed in real time by micro-gravimetry. The fabricated lipidpolyelectrolyte composite structures on top of multilayer coated colloidal particles were characterized by flow cytometry and imaging techniques. Lipid diffusion over the macroscopic scale was quantified by fluorescence recovery after photobleaching, and the diffusion was related to layer connectivity. The phospholipidpolyelectrolyte binding mechanism was investigated by infrared spectroscopy. A strong interaction of polyelectrolyte primary amino groups with phosphate and carboxyl groups of the phospholipids, leading to dehydration, was observed. Long-range electrostatic attraction was proven to be essential for vesicle spreading and rupture. Fusion of lipid patches into a homogeneous bilayer required lateral mobility of the lipids on the polyelectrolyte support. The binding of amino groups to the phosphate group of the zwitterionic lipids was too weak to induce vesicle spreading, but sufficient for strong adsorption. Only the mixture of phosphatidylcholine and phosphatidylserine resulted in the spontaneous formation of bilayers on polyelectrolyte multilayers. The adsorption of phospholipids onto multilayers displaying quarternary ammonium polymers produced a novel 3D lipid polyelectrolyte structure on colloidal particles.

Graphical abstract: Lipid layers on polyelectrolyte multilayer supports

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Publication details

The article was received on 07 Apr 2008, accepted on 30 Jun 2008 and first published on 18 Aug 2008


Article type: Paper
DOI: 10.1039/B805754K
Soft Matter, 2008,4, 2245-2258

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    Lipid layers on polyelectrolyte multilayer supports

    M. Fischlechner, M. Zaulig, S. Meyer, I. Estrela-Lopis, L. Cuéllar, J. Irigoyen, P. Pescador, M. Brumen, P. Messner, S. Moya and E. Donath, Soft Matter, 2008, 4, 2245
    DOI: 10.1039/B805754K

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