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Issue 33, 2020
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Triazole-based osmium(ii) complexes displaying red/near-IR luminescence: antimicrobial activity and super-resolution imaging

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Abstract

Cellular uptake, luminescence imaging and antimicrobial activity against clinically relevant methicillin-resistant S. aureus (MRSA) bacteria are reported. The osmium(II) complexes [Os(N^N)3]2+ (N^N = 1-benzyl-4-(pyrid-2-yl)-1,2,3-triazole (12+); 1-benzyl-4-(pyrimidin-2-yl)-1,2,3-triazole (22+); 1-benzyl-4-(pyrazin-2-yl)-1,2,3-triazole (32+)) were prepared and isolated as the chloride salts of their meridional and facial isomers. The complexes display prominent spin-forbidden ground state to triplet metal-to-ligand charge transfer (3MLCT) state absorption bands enabling excitation as low as 600 nm for fac/mer-32+ and observation of emission in aqueous solution in the deep-red/near-IR regions of the spectrum. Cellular uptake studies within MRSA cells show antimicrobial activity for 12+ and 22+ with greater toxicity for the meridional isomers in each case and mer-12+ showing the greatest potency (32 μg mL−1 in defined minimal media). Super-resolution imaging experiments demonstrate binding of mer- and fac-12+ to bacterial DNA with high Pearson's colocalisation coefficients (up to 0.95 using DAPI). Phototoxicity studies showed the complexes exhibited a higher antimicrobial activity upon irradiation with light.

Graphical abstract: Triazole-based osmium(ii) complexes displaying red/near-IR luminescence: antimicrobial activity and super-resolution imaging

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Supplementary files

Article information


Submitted
28 Jun 2020
Accepted
06 Aug 2020
First published
07 Aug 2020

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2020,11, 8928-8935
Article type
Edge Article

Triazole-based osmium(II) complexes displaying red/near-IR luminescence: antimicrobial activity and super-resolution imaging

K. L. Smitten, P. A. Scattergood, C. Kiker, J. A. Thomas and P. I. P. Elliott, Chem. Sci., 2020, 11, 8928
DOI: 10.1039/D0SC03563G

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