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Issue 40, 2019
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Site-specific C-terminal dinitrophenylation to reconstitute the antibody Fc functions for nanobodies

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Abstract

Nanobodies are a class of camelid-derived single-domain antibodies that have many potential advantages over conventional antibodies and have been utilized to develop new therapeutic strategies for cancer and other diseases. However, nanobodies lack the Fc region of a conventional antibody, which possesses many functions, e.g., eliciting antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC), essential for effective immunotherapy. The small molecular size of nanobodies also leads to poor pharmacokinetics, such as short in vivo half-life. To address these deficiencies, an endogenous antibody-based strategy to reconstitute the Fc functions for nanobodies was developed. As a proof-of-principle, an anti-human EGFR nanobody, 7D12, was selected to conduct C-terminal modification with the dinitrophenyl (DNP) hapten through Sortase A-mediated site-specific ligation. It was expected that the DNP motif would recruit endogenous human anti-DNP antibodies to indirectly reinstate the Fc functions. The resultant nanobody-DNP conjugates were shown to exhibit specific and high affinity binding to human EGFR expressed on target cancer cells. It was further proved that in the presence of anti-DNP antibody, these conjugates could mediate potent ADCC and CDC in vitro and exhibit significantly elongated half-life in vivo. Ultimately, it was proven in severe combined immunodeficiency (SCID) mice that treatment with the nanobody 7D12-DNP conjugate and anti-DNP mouse serum could inhibit xenograft tumor growth efficiently. In view of the abundance of anti-DNP and other endogenous antibodies in the human blood system, this could be a generally applicable approach employed to reconstitute the Fc functions for nanobodies and develop nanobody-based cancer immunotherapy and other therapies.

Graphical abstract: Site-specific C-terminal dinitrophenylation to reconstitute the antibody Fc functions for nanobodies

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Supplementary files

Article information


Submitted
02 Aug 2019
Accepted
19 Aug 2019
First published
28 Aug 2019

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2019,10, 9331-9338
Article type
Edge Article

Site-specific C-terminal dinitrophenylation to reconstitute the antibody Fc functions for nanobodies

H. Hong, Z. Zhou, K. Zhou, S. Liu, Z. Guo and Z. Wu, Chem. Sci., 2019, 10, 9331
DOI: 10.1039/C9SC03840J

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    [Original citation] - Published by The Royal Society of Chemistry.

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