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Issue 31, 2019
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Dynamic colloidal nanoparticle assembly triggered by aptamer–receptor interactions on live cell membranes

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Abstract

Cells use dynamic systems such as enzyme cascades and signaling networks to control cellular functions. Synthetic dynamic systems that can be target-responsive have great potential to be applied for biomedical applications but the operation of such dynamic systems in complex cellular environments remains challenging. Here, we engineered an aptamer and DNA displacement reaction-based dynamic system that can transform its nanostructure in response to the epithelial cell adhesion molecule (EpCAM) on live cell membranes. The dynamic system consisted of a core nanoparticle and small satellite nanoparticles. With the modifications of different DNA hairpin strands and swing arm strands partially hybridized with an aptamer that specifically recognizes the EpCAM, the two separated particles can dynamically assemble into a core–satellite assembly by aptamer–receptor interactions on the cell membrane surface. The structural change of the system from separated particles to a core–satellite assembly generated plasmonic coupled hot spots for surface-enhanced Raman scattering (SERS) for sensitively capturing the dynamic structural change of the nanoassembly in the cellular environment. These concepts provide strategies for engineering dynamic nanotechnology systems for biological and biomedical applications in complex biological environments.

Graphical abstract: Dynamic colloidal nanoparticle assembly triggered by aptamer–receptor interactions on live cell membranes

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Supplementary files

Article information


Submitted
03 Jun 2019
Accepted
21 Jun 2019
First published
28 Jun 2019

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2019,10, 7466-7471
Article type
Edge Article

Dynamic colloidal nanoparticle assembly triggered by aptamer–receptor interactions on live cell membranes

L. Yang, L. Meng, J. Song, Y. Xiao, R. Wang, H. Kang and D. Han, Chem. Sci., 2019, 10, 7466
DOI: 10.1039/C9SC02693B

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

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    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
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    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
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    [Original citation] - Published by The Royal Society of Chemistry.

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