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Issue 29, 2019
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Aza-proline effectively mimics l-proline stereochemistry in triple helical collagen

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Abstract

The prevalence of L-amino acids in biomolecules has been shown to have teleological importance in biomolecular structure and self-assembly. Recently, biophysical studies have demonstrated that natural L-amino acids can be replaced with non-natural achiral aza-amino acids in folded protein structures such as triple helical collagen. However, the structural consequences of achiral aza-amino acid incorporation has not been elucidated in the context of any relevant folded biomolecule. Herein, we use X-ray crystallography to provide the first atomic resolution crystal structure of an achiral aza-amino acid residue embedded within a folded protein structure, definitively illustrating that achiral aza-proline has the capacity to effectively mimic the stereochemistry of natural amino acids within the context of triple helical collagen. We further corroborate this finding with density functional theory computational analysis showing that the natural L-amino acid stereochemistry for aza-proline is energetically favored when arranged in the aza-proline-hydroxyproline-glycine motif. In addition to providing fundamental insight into peptide and protein structure, the incorporation of achiral stereochemical mimics such as aza-amino acids could have far reaching impacts in areas ranging from synthetic materials to drug design.

Graphical abstract: Aza-proline effectively mimics l-proline stereochemistry in triple helical collagen

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Supplementary files

Article information


Submitted
05 May 2019
Accepted
19 Jun 2019
First published
21 Jun 2019

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2019,10, 6979-6983
Article type
Edge Article

Aza-proline effectively mimics L-proline stereochemistry in triple helical collagen

A. J. Kasznel, T. Harris, N. J. Porter, Y. Zhang and David M. Chenoweth, Chem. Sci., 2019, 10, 6979
DOI: 10.1039/C9SC02211B

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