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Issue 10, 2019
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Programmable intracellular DNA biocomputing circuits for reliable cell recognitions

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Abstract

Dynamic nucleic acid-based biocircuits have spurred substantial research efforts for diagnosis or biomedical applications at the molecular level; nevertheless, it still remains a challenge to design programmable molecular circuit devices for autonomous and accurate diagnosis of low abundance biomolecules in a complex intracellular environment. Herein, a reconfigurable hybridization-based chain reaction is introduced to assemble modular biocomputing circuits that include a general sensing module and a versatile processing module. By modular sensing module design, we realized multiple endogenous miRNA-initiated biocomputing operations, including binary logic gates (OR, AND, INHIBIT and XOR), and more advanced concatenated logic circuits (XOR-AND, XOR-INHIBIT, and XOR-OR) in different living cells. The sensing module transduces the primary miRNA sensing event into an intermediate trigger for activating the processing module that further transduces the specific analyte recognition pattern into an amplified fluorescence readout. Based on an appropriate selection of multiple miRNA analytes, various miRNA expression patterns could be utilized for sensitive and selective cell discriminations. The inherent synergistically accelerated recognition and hybridization features of our biocomputing systems contribute to the amplified detection of multiplex endogenous miRNAs in living cells, thus providing an efficient toolbox for more accurate diagnosis and programmable therapeutics.

Graphical abstract: Programmable intracellular DNA biocomputing circuits for reliable cell recognitions

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Publication details

The article was received on 22 Nov 2018, accepted on 15 Jan 2019 and first published on 15 Jan 2019


Article type: Edge Article
DOI: 10.1039/C8SC05217D
Chem. Sci., 2019,10, 2989-2997
  • Open access: Creative Commons BY-NC license
    All publication charges for this article have been paid for by the Royal Society of Chemistry

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    Programmable intracellular DNA biocomputing circuits for reliable cell recognitions

    X. Gong, J. Wei, J. Liu, R. Li, X. Liu and F. Wang, Chem. Sci., 2019, 10, 2989
    DOI: 10.1039/C8SC05217D

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