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Issue 3, 2019
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Two-photon fluorescence imaging reveals a Golgi apparatus superoxide anion-mediated hepatic ischaemia-reperfusion signalling pathway

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Abstract

Hepatic ischaemia-reperfusion (IR) injury is mainly attributed to a burst of reactive oxygen species (ROS) that attack biological macromolecules and lead to cell death. The superoxide anion (O2˙) is the first ROS to be generated and triggers the production of other ROS; thus, explorations of the role of O2˙ in the IR process are meaningful. Meanwhile, the Golgi apparatus generates O2˙via Golgi-associated proteins, which might play an essential role in IR injury. However, the molecular mechanism by which O2˙ from the Golgi apparatus regulates hepatic IR injury is unclear. Therefore, to solve this problem, a two-photon (TP) excited fluorescence probe (CCA) was designed and prepared for the reversible detection of O2˙ in the Golgi apparatus. With the assistance of TP fluorescence microscopy, we observed a substantial increase in the levels of O2˙ in the Golgi apparatus of an IR mouse liver for the first time, as well as increased caspase-2 activity and apoptosis. Furthermore, we found that the tumour necrosis factor (TNF-α) functions as a positive mediator of O2˙ generation. Based on these data, we identified the potential signalling pathway in the Golgi that mediates O2˙ fluctuations in IR mice and revealed the related molecular mechanisms; we also provide a new target for treating IR injury.

Graphical abstract: Two-photon fluorescence imaging reveals a Golgi apparatus superoxide anion-mediated hepatic ischaemia-reperfusion signalling pathway

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Publication details

The article was received on 03 Sep 2018, accepted on 01 Nov 2018 and first published on 06 Nov 2018


Article type: Edge Article
DOI: 10.1039/C8SC03917H
Chem. Sci., 2019,10, 879-883
  • Open access: Creative Commons BY-NC license
    All publication charges for this article have been paid for by the Royal Society of Chemistry

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    Two-photon fluorescence imaging reveals a Golgi apparatus superoxide anion-mediated hepatic ischaemia-reperfusion signalling pathway

    W. Zhang, J. Zhang, P. Li, J. Liu, D. Su and B. Tang, Chem. Sci., 2019, 10, 879
    DOI: 10.1039/C8SC03917H

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