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Issue 42, 2018
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Fluorescent probes guided by a new practical performance regulation strategy to monitor glutathione in living systems

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Abstract

Glutathione (GSH) plays an important role in the body's biochemical defense system, and the detection of GSH in a physiological system is an important tool for understanding redox homeostasis. Protection–deprotection strategies have proven to be the most reliable, among existing detection methods. However, the understanding of how various electronic and steric effects influence a probe's ability to recognize a substrate is still lacking. In this study, we have analyzed various substituent effects on a GSH probe template via theoretical calculations and constructed the performance regulation and control strategy for this kind of probe. We then developed a series of guided probes using eighteen different acrylic ester derivatives to mask the fluorescence of fluorescein. The optical performance differences between the guided probes strongly supported the applicability of our proposed guiding strategy. Moreover, the positively guided probes are excellent for imaging GSH distribution in living cells and mice.

Graphical abstract: Fluorescent probes guided by a new practical performance regulation strategy to monitor glutathione in living systems

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Supplementary files

Article information


Submitted
01 Aug 2018
Accepted
19 Sep 2018
First published
25 Sep 2018

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2018,9, 8065-8070
Article type
Edge Article

Fluorescent probes guided by a new practical performance regulation strategy to monitor glutathione in living systems

M. She, Z. Wang, T. Luo, B. Yin, P. Liu, J. Liu, F. Chen, S. Zhang and J. Li, Chem. Sci., 2018, 9, 8065
DOI: 10.1039/C8SC03421D

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