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Issue 42, 2018
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On-demand quantitative SERS bioassays facilitated by surface-tethered ratiometric probes

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Abstract

Reliable and user-friendly sensing of target analytes in complex biofluids is of fundamental importance to biological science and medicine. Surface-enhanced Raman spectroscopy (SERS) has proven to be capable of detecting molecules with high sensitivity, but achieving robust quantitative detection remains a challenge mainly because of the severe signal fluctuation at electromagnetic hot spots. Here, we describe an on-demand and quantitative SERS strategy for metabolite profiling based on a chip-based sensing device that adopts stable and surface-tethered small-molecule probes as Raman reporters. These probes with a ratiometric response allow for sensitive and reproducible SERS detection by offering an internal calibration to correct the signal fluctuation caused by the spatiotemporal variation of assay conditions. Meanwhile, the chip-based sensing scheme makes time-separated on-demand detection possible. Ultimately, due to the flexibility in choosing diverse ratiometric Raman probes, we expect the proposed quantitative SERS sensing concept to be useful for studies in the fields of cell biology and clinical diagnosis.

Graphical abstract: On-demand quantitative SERS bioassays facilitated by surface-tethered ratiometric probes

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Supplementary files

Article information


Submitted
24 Jul 2018
Accepted
27 Aug 2018
First published
31 Aug 2018

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2018,9, 8089-8093
Article type
Edge Article

On-demand quantitative SERS bioassays facilitated by surface-tethered ratiometric probes

K. Zhang, Y. Wang, M. Wu, Y. Liu, D. Shi and B. Liu, Chem. Sci., 2018, 9, 8089
DOI: 10.1039/C8SC03263G

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

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    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
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    [Original citation] - Published by The Royal Society of Chemistry.

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