Issue 29, 2018

Membrane glycomics reveal heterogeneity and quantitative distribution of cell surface sialylation

Abstract

Given that unnatural sugar expression is metabolically achieved, the kinetics and disposition of incorporation can lend insight into the temporal and localization preferences of sialylation across the cell surface. However, common detection schemes lack the ability to detail the molecular diversity and distribution of target moieties. Here we employed a mass spectrometric approach to trace the placement of azido sialic acids on membrane glycoconjugates, which revealed substantial variations in incorporation efficiencies between N-/O-glycans, glycosites, and glycosphingolipids. To further explore the propensity for sialylation, we subsequently mapped the native glycome of model epithelial cell surfaces and illustrate that while glycosylation sites span broadly across the extracellular region, a higher number of heterogeneous glycoforms occur on sialylated sites closest to the transmembrane domain. Beyond imaging techniques, this integrative approach provides unprecedented details about the frequency and structure-specific distribution of cell surface sialylation, a critical feature that regulates cellular interactions and homeostatic pathways.

Graphical abstract: Membrane glycomics reveal heterogeneity and quantitative distribution of cell surface sialylation

Supplementary files

Article information

Article type
Edge Article
Submitted
24 Apr 2018
Accepted
26 Jun 2018
First published
27 Jun 2018
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2018,9, 6271-6285

Membrane glycomics reveal heterogeneity and quantitative distribution of cell surface sialylation

D. D. Park, G. Xu, M. Wong, C. Phoomak, M. Liu, N. E. Haigh, S. Wongkham, P. Yang, E. Maverakis and C. B. Lebrilla, Chem. Sci., 2018, 9, 6271 DOI: 10.1039/C8SC01875H

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