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Issue 4, 2018
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Design of DNA nanostructure-based interfacial probes for the electrochemical detection of nucleic acids directly in whole blood

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Abstract

Here we report a robust and sensitive DNA nanostructure-based electrochemical (E-nanoDNA) sensor that utilizes tetrahedral DNA nanostructures (TDNs) as an interfacial probe to detect biomolecules in a single-step procedure. In this study, we have firstly demonstrated that the use of TDNs can significantly suppress electrochemical background signals compared to traditional linear DNA probes upon introduction of base mismatches in the edges of TDNs. After further optimization of the two functional strands in the TDNs, quantitative, one-step detection of DNA can be achieved in the picomolar range in less than 10 min, and directly in complex media. Moreover, the baseline drift of this biosensor can be greatly decreased even after several hours in flowing whole blood in vitro, which suggests that the sensor holds potential to be employed in live animals. Furthermore, through replacing functional strands with aptamers or other DNA elements, this E-nanoDNA sensor can be easily used to probe various analytes, broadening the application range of the proposed sensor.

Graphical abstract: Design of DNA nanostructure-based interfacial probes for the electrochemical detection of nucleic acids directly in whole blood

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Supplementary files

Article information


Submitted
27 Oct 2017
Accepted
26 Nov 2017
First published
28 Nov 2017

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2018,9, 979-984
Article type
Edge Article

Design of DNA nanostructure-based interfacial probes for the electrochemical detection of nucleic acids directly in whole blood

C. Li, X. Hu, J. Lu, X. Mao, Y. Xiang, Y. Shu and G. Li, Chem. Sci., 2018, 9, 979
DOI: 10.1039/C7SC04663D

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