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Issue 11, 2017
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Simultaneous quantification of multiple endogenous biothiols in single living cells by plasmonic Raman probes

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Abstract

Intracellular biothiols mediate many important physiological and pathological processes. Due to their low content and competing thiol-reactivity, it is still an unmet challenge to quantify them within a complicated intracellular environment. Herein, we demonstrated a strategy to discriminate three biothiols, i.e. cysteine (Cys), homo-cysteine (Hcy) and glutathione (GSH), and quantify their concentrations within single living cells, using one platform of Raman probe. By monitoring the reaction kinetics of biothiols with Raman probes and discriminating their products with a quantitative principal component analysis (qPCA) method, these three biothiols could be simultaneously quantified in both cell lysis and single living cells. The concentrations of Cys, Hcy and GSH in single Hela cells were 158 ± 19 μM, 546 ± 67 μM and 5.07 ± 0.62 mM, respectively, which gives the precise concentrations of these three biothiols at a single cell level for the first time. This method provides a general strategy for discriminating each component from a mixed system and has potential for quantifying any biomolecules within an in vitro or in vivo biological environment.

Graphical abstract: Simultaneous quantification of multiple endogenous biothiols in single living cells by plasmonic Raman probes

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Supplementary files

Article information


Submitted
24 Jul 2017
Accepted
28 Aug 2017
First published
29 Aug 2017

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2017,8, 7582-7587
Article type
Edge Article

Simultaneous quantification of multiple endogenous biothiols in single living cells by plasmonic Raman probes

S. Li, Q. Guan, M. Zheng, Y. Wang, D. Ye, B. Kang, J. Xu and H. Chen, Chem. Sci., 2017, 8, 7582
DOI: 10.1039/C7SC03218H

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