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Issue 5, 2016
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Metal-responsive structural transformation between artificial DNA duplexes and three-way junctions

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Abstract

DNA three-way junctions (3WJs) are essential structural motifs for DNA nanoarchitectures and DNA-based materials. We report herein a metal-responsive structural transformation between DNA duplexes and 3WJs using artificial oligonucleotides modified with a 2,2′-bipyridine (bpy) ligand. A mixture of bpy-modified DNA strands and natural complementary strands were self-assembled exclusively into duplexes without any transition metal ions, while they formed 3WJs in the presence of NiII ions. This transformation was induced by the formation of an interstrand NiII(bpy)3 complex, which served as a template for the 3WJ assembly. Altering the amount and identity of the metal ion regulated the 3WJ induction efficiency. Removal of the metal using EDTA quantitatively regenerated the duplexes. The metal-dependent structural conversion shown here has many potential applications in the development of stimuli-responsive DNA materials.

Graphical abstract: Metal-responsive structural transformation between artificial DNA duplexes and three-way junctions

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Supplementary files

Article information


Submitted
26 Jan 2016
Accepted
15 Feb 2016
First published
16 Feb 2016

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2016,7, 3006-3010
Article type
Edge Article

Metal-responsive structural transformation between artificial DNA duplexes and three-way junctions

Y. Takezawa, S. Yoneda, J. H. A. Duprey, T. Nakama and M. Shionoya, Chem. Sci., 2016, 7, 3006
DOI: 10.1039/C6SC00383D

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