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Issue 7, 2016
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A FRET probe with AIEgen as the energy quencher: dual signal turn-on for self-validated caspase detection

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Abstract

The accurate detection of biological substances is highly desirable to study various biological processes and evaluate disease progression. Herein, we report a self-validated fluorescent probe which is composed of a coumarin fluorophore as the energy donor and a fluorogen with aggregation-induced emission characteristics (AIEgen) as the energy quencher linked through a caspase-3 specific peptide substrate. Unlike the traditionally widely studied fluorescence resonance energy transfer (FRET) probes, our new generation of FRET probe is non-fluorescent itself due to the energy transfer as well as the dissipation of the acceptor energy through the free molecular motion of AIEgen. Upon interaction with caspase-3, the probe displays strong green and red fluorescent signals synchronously due to the separation of the donor–quencher and aggregation of the released AIEgen. The fluorescence turn-on with dual signal amplification allows real-time and self-validated enzyme detection with a high signal-to-background ratio, providing a good opportunity to accurately monitor various biological processes in a real-time manner.

Graphical abstract: A FRET probe with AIEgen as the energy quencher: dual signal turn-on for self-validated caspase detection

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Article information


Submitted
06 Jan 2016
Accepted
16 Mar 2016
First published
16 Mar 2016

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2016,7, 4245-4250
Article type
Edge Article

A FRET probe with AIEgen as the energy quencher: dual signal turn-on for self-validated caspase detection

Y. Yuan, R. Zhang, X. Cheng, S. Xu and B. Liu, Chem. Sci., 2016, 7, 4245
DOI: 10.1039/C6SC00055J

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