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Issue 3, 2016
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Using modified aptamers for site specific protein–aptamer conjugations

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Abstract

Conjugation of DNA to defined locations on a protein surface will be a powerful tool for positioning functional groups and molecules in biological and biomedical studies. However, tagging protein with DNA is challenging in physiological environments, and requires a bioorthogonal approach. Here, we report a chemical solution to selectively conjugate DNA aptamers with a protein by protein–aptamer template (PAT)-directed reactions. Since protein–aptamer interactions are bioorthogonal, we exploit the PAT as a unique platform for specific DNA–protein cross-linking. We develop a series of modified oligonucleotides for PAT-directed reactions and find an F-carboxyl group as a suitable functionality for selective and site-specific conjugation. The functionality is incorporated into aptamers in our F-carboxyl phosphoramidite with an easy synthesis. We also demonstrate the necessity of a linker between the reactive functionality and the aptamer sequences.

Graphical abstract: Using modified aptamers for site specific protein–aptamer conjugations

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Supplementary files

Article information


Submitted
20 Jul 2015
Accepted
19 Nov 2015
First published
10 Dec 2015

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2016,7, 2157-2161
Article type
Edge Article
Author version available

Using modified aptamers for site specific protein–aptamer conjugations

R. Wang, D. Lu, H. Bai, C. Jin, G. Yan, M. Ye, L. Qiu, R. Chang, C. Cui, H. Liang and W. Tan, Chem. Sci., 2016, 7, 2157
DOI: 10.1039/C5SC02631H

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    [Original citation] - Published by The Royal Society of Chemistry.

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