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Issue 12, 2015
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Visualizing changes in mitochondrial Mg2+ during apoptosis with organelle-targeted triazole-based ratiometric fluorescent sensors

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Abstract

Magnesium is one of the most abundant metals in cells and is essential for a wide range of cellular processes. Magnesium imbalance has been linked to a variety of diseases, but the scarcity of sensors suitable for detection of Mg2+ with subcellular resolution has hampered the study of compartmentalization and mobilization of this ion in the context of physiological and pathological processes. We report herein a family of fluorescent probes for targeted detection of free Mg2+ in specific intracellular organelles, and its application in the study of programmed cell death. The new sensors feature a triazole unit that plays both structural and electronic roles by serving as an attachment group for targeting moieties, and modulating a possible internal charge transfer process for ratiometric ion sensing. A probe decorated with an alkylphosphonium group was employed for the detection of mitochondrial Mg2+ in live HeLa cells, providing the first direct observation of an increase in free Mg2+ levels in this organelle in the early stages of Staurosporine-induced apoptosis.

Graphical abstract: Visualizing changes in mitochondrial Mg2+ during apoptosis with organelle-targeted triazole-based ratiometric fluorescent sensors

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Publication details

The article was received on 07 Jul 2015, accepted on 15 Oct 2015 and first published on 28 Oct 2015


Article type: Edge Article
DOI: 10.1039/C5SC02442K
Chem. Sci., 2015,6, 6841-6846
  • Open access: Creative Commons BY-NC license
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    Visualizing changes in mitochondrial Mg2+ during apoptosis with organelle-targeted triazole-based ratiometric fluorescent sensors

    G. Zhang, J. J. Gruskos, M. S. Afzal and D. Buccella, Chem. Sci., 2015, 6, 6841
    DOI: 10.1039/C5SC02442K

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