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Issue 7, 2015
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Target-driven DNA association to initiate cyclic assembly of hairpins for biosensing and logic gate operation

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Abstract

A target-driven DNA association was designed to initiate cyclic assembly of hairpins, which led to an enzyme-free amplification strategy for detection of a nucleic acid or aptamer substrate and flexible construction of logic gates. The cyclic system contained two ssDNA (S1 and S2) and two hairpins (H1 and H2). These ssDNA could co-recognize the target to produce an S1–target–S2 structure, which brought their toehold and branch-migration domains into close proximity to initiate the cyclic assembly of hairpins. The assembly product further induced the dissociation of a double-stranded probe DNA (Q:F) via toehold-mediated strand displacement to switch the fluorescence signal. This method could detect DNA and ATP as model analytes down to 21.6 pM and 38 nM, respectively. By designing different DNA input strands, the “AND”, “INHIBIT” and “NAND” logic gates could be activated to achieve the output signal. The proposed biosensing and logic gate operation platform showed potential applications in disease diagnosis.

Graphical abstract: Target-driven DNA association to initiate cyclic assembly of hairpins for biosensing and logic gate operation

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The article was received on 05 Apr 2015, accepted on 11 May 2015 and first published on 12 May 2015


Article type: Edge Article
DOI: 10.1039/C5SC01215E
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Chem. Sci., 2015,6, 4318-4323
  • Open access: Creative Commons BY license
    All publication charges for this article have been paid for by the Royal Society of Chemistry

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    Target-driven DNA association to initiate cyclic assembly of hairpins for biosensing and logic gate operation

    Y. Guo, J. Wu and H. Ju, Chem. Sci., 2015, 6, 4318
    DOI: 10.1039/C5SC01215E

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

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